Journal: NPJ Precision Oncology

Article Title: Single-cell analysis reveals CD34 + CD90 + endothelial cells promote tumor metastasis in gallbladder cancer

doi: 10.1038/s41698-025-01040-2

Figure Lengend Snippet: A Flow cytometry was used to detect CD34 + CD90 + ECs in 2 GBC tissues (PE: CD34, FITC: CD90). B Representative multiple immune staining images of CD34 and CD90 in GBC tissues. C Immune staining of CD34 and CD90 on GBC tissue microarray. D Analysis of the correlation between CD34 + or CD34 + CD90 + cell contents and patient survival prognosis and clinical characteristics. OS, overall survival time.

Article Snippet: Subsequently, FITC-conjugated CD90 antibody was added to the sorted CD34 + endothelial cells, and an EasySepTM Release Human FITC Positive Selection Kit (STEMCELL Technologies) was used to isolate CD34 + CD90 + endothelial cells.

Techniques: Flow Cytometry, Staining, Microarray

Journal: Malaria Journal

Article Title: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

doi: 10.1186/s12936-018-2547-3

Figure Lengend Snippet: List of fluorophore-conjugated antibodies

Article Snippet: The magnetic B and Th cell isolation from whole blood was carried out using the EasySepTM HLA Chimerism Whole Blood CD19 Positive Selection Kit (Stemcell Technologies) and the EasySepTM Human Whole Blood CD4 Positive Selection Kit (Stemcell Technologies) [ , ].

Techniques:

Journal: Malaria Journal

Article Title: High purity high yield tandem B and T helper cell isolation for qRT-PCR analysis suitable for basically equipped laboratories

doi: 10.1186/s12936-018-2547-3

Figure Lengend Snippet: Flow cytometry analysis of purity and efficiency of B and Th cell isolation. Shown is a representative analysis of three independent isolations. a Flow cytometry analysis of whole blood after erythrocyte lysis (= before B cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper right: total T cells (CD3+) versus B cells (CD19+). Lower left: total T cells (CD3+) versus Th cells (CD3+CD4+). b Flow cytometry analysis of isolated CD19+ B cells (= positive fraction after B cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells. c Flow cytometry analysis of isolated CD4+ Th cells (= positive fraction after Th cell isolation). Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells. d Flow cytometry analysis of the negative fraction after Th cell isolation. Upper left: gating of live lymphocytes. Lower left: gating of single cells. Upper middle: total T cells (CD3+) versus B cells (CD19+). Lower middle: total T cells (CD3+) versus Th cells (CD3+CD4+). Upper right: histogram of CD19 positive cells. Lower right: histogram of CD4 positive cells

Article Snippet: The magnetic B and Th cell isolation from whole blood was carried out using the EasySepTM HLA Chimerism Whole Blood CD19 Positive Selection Kit (Stemcell Technologies) and the EasySepTM Human Whole Blood CD4 Positive Selection Kit (Stemcell Technologies) [ , ].

Techniques: Flow Cytometry, Cell Isolation, Lysis, Isolation